ELISA/assay

Porcine Brain Natriuretic Peptide ELISA Kit

MBS734047

48-Strip-Wells

470 USD

ELISA Kit

Porcine Brain Natriuretic Peptide ELISA Kit

Brain Natriuretic Peptide

brain natriuretic peptide; Brain natriuretic peptide; brain natriuretic peptide; B-type natriuretic peptide

BNP

nppb; nppb; bnp; bnp

ANFB_TAKRU

Porcine

This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BNP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Neurobiology

Intended Uses: This BNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine BNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay BNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BNP antibody and an BNP-HRP conjugate. The assay sample and buffer are incubated together with BNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BNP concentration since BNP from samples and BNP-HRP conjugate compete for the anti-BNP antibody binding site. Since the number of sites is limited, as more sites are occupied by BNP from the sample, fewer sites are left to bind BNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BNP concentration in each sample is interpolated from this standard curve.

74096009

NP_001027860.1

NM_001032688.1

14,746 Da

48-Strip-Wells

470 USD

96-Strip-Wells

675