catalog number :
MBS733932
products type :
ELISA Kit
products full name :
Human Epididymal Protein 4 (HE4) ELISA Kit
products short name :
Epididymal Protein 4 (HE4)
specificity :
This assay has high sensitivity and excellent specificity for detection of HE4. No significant cross-reactivity or interference between HE4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HE4 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pmol/ml.
other info2 :
Intended Uses: This HE4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HE4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: HE4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HE4 antibody and an HE4-HRP conjugate. The assay sample and buffer are incubated together with HE4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HE4 concentration since HE4 from samples and HE4-HRP conjugate compete for the anti-HE4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HE4 from the sample, fewer sites are left to bind HE4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HE4 concentration in each sample is interpolated from this standard curve.