ELISA/assay

Bovine Interleukin 10 ELISA Kit

MBS733926

48-Strip-Wells

440 USD

ELISA Kit

Bovine Interleukin 10 ELISA Kit

[Interleukin 10]

[IL-10; IL-10]

[IL-10]

[SPPV_03]

Bovine

This assay has high sensitivity and excellent specificity for detection of IL-1. No significant cross-reactivity or interference between IL-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This IL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-1 antibody and an IL-1-HRP conjugate. The assay sample and buffer are incubated together with IL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1 concentration since IL-1 from samples and IL-1-HRP conjugate compete for the anti-IL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1 from the sample, fewer sites are left to bind IL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1 concentration in each sample is interpolated from this standard curve.

21492460

NP_659579.1

NC_004002.1

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415