ELISA/assay

Mouse Metallothionein-3 ELISA Kit

MBS733924

48-Strip-Wells

470 USD

ELISA Kit

Mouse Metallothionein-3 ELISA Kit

Metallothionein-3

metallothionein-3; Metallothionein-3; metallothionein-3; MT-3; MT-III; metallothionein-III; growth inhibitory factor; metallothionein 3 (growth inhibitory factor (neurotrophic)); metallothionein 3; GIFB; GIF; Growth inhibitory factor; Metallothionein-III; MT-III

MT-3

MT3; MT3; GIF; GIFB; GRIF; ZnMT3; MT-3; GIF; MT-III

MT3_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Microbiology

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This MET3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MET3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MET3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MET3 antibody and an MET3-HRP conjugate. The assay sample and buffer are incubated together with MET3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MET3 concentration since MET3 from samples and MET3-HRP conjugate compete for the anti-MET3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MET3 from the sample, fewer sites are left to bind MET3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MET3 concentration in each sample is interpolated from this standard curve.

5174762

NP_005945.1

NM_005954.2

6,927 Da

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; inclusion body; intracellular. Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; cadmium ion binding; protein kinase activator activity. Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of axon extension; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; leptin-mediated signaling pathway; astrocyte development; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; response to hypoxia; energy reserve metabolic process; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675