product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Metallothionein 3 ELISA Kit
catalog :
MBS733923
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS733923
products type :
ELISA Kit
products full name :
Human Metallothionein 3 ELISA Kit
products short name :
Metallothionein 3
other names :
metallothionein-3; Metallothionein-3; metallothionein-3; MT-3; MT-III; metallothionein-III; growth inhibitory factor; metallothionein 3 (growth inhibitory factor (neurotrophic)); metallothionein 3; GIFB; GIF; Growth inhibitory factor; Metallothionein-III; MT-III
products gene name :
MT 3
other gene names :
MT3; MT3; GIF; GIFB; GRIF; ZnMT3; MT-3; GIF; MT-III
uniprot entry name :
MT3_HUMAN
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of MT3. No significant cross-reactivity or interference between MT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MT3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This MT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Microbiology
products description :
. Principle of the assay: MT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT3 antibody and an MT3-HRP conjugate. The assay sample and buffer are incubated together with MT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT3 concentration since MT3 from samples and MT3-HRP conjugate compete for the anti-MT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT3 from the sample, fewer sites are left to bind MT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT3 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
5174762
ncbi acc num :
NP_005945.1
ncbi gb acc num :
NM_005954.2
ncbi mol weight :
6,927 Da
uniprot summary :
MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; intracellular; inclusion body. Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; protein kinase activator activity; cadmium ion binding. Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of caspase activity; negative regulation of axon extension; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; astrocyte development; leptin-mediated signaling pathway; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; response to hypoxia; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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