Human Metallothionein 3 ELISA Kit | MyBioSource MBS733923 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Metallothionein 3 ELISA Kit

catalog :

MBS733923

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733923

products type :

ELISA Kit

products full name :

Human Metallothionein 3 ELISA Kit

products short name :

Metallothionein 3

other names :

metallothionein-3; Metallothionein-3; metallothionein-3; MT-3; MT-III; metallothionein-III; growth inhibitory factor; metallothionein 3 (growth inhibitory factor (neurotrophic)); metallothionein 3; GIFB; GIF; Growth inhibitory factor; Metallothionein-III; MT-III

products gene name :

MT 3

other gene names :

MT3; MT3; GIF; GIFB; GRIF; ZnMT3; MT-3; GIF; MT-III

uniprot entry name :

MT3_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of MT3. No significant cross-reactivity or interference between MT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MT3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This MT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Microbiology

products description :

. Principle of the assay: MT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT3 antibody and an MT3-HRP conjugate. The assay sample and buffer are incubated together with MT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT3 concentration since MT3 from samples and MT3-HRP conjugate compete for the anti-MT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT3 from the sample, fewer sites are left to bind MT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5174762

ncbi acc num :

NP_005945.1

ncbi gb acc num :

NM_005954.2

ncbi mol weight :

6,927 Da

uniprot summary :

MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; intracellular; inclusion body. Molecular Function: antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; protein kinase activator activity; cadmium ion binding. Biological Process: positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of caspase activity; negative regulation of axon extension; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; activation of protein kinase B; cellular zinc ion homeostasis; zinc ion transport; regulation of protein amino acid glycosylation; astrocyte development; leptin-mediated signaling pathway; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; response to hypoxia; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage