product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Protease Activated Receptor 1 ELISA Kit
catalog :
MBS733919
quantity :
96 Strip Wells
price :
675 USD
more info or order :
product information
catalog number :
MBS733919
products type :
ELISA Kit
products full name :
Human Protease Activated Receptor 1 ELISA Kit
products short name :
Protease Activated Receptor 1
other names :
sex hormone-binding globulin; Sex hormone-binding globulin; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin
products gene name :
PAR1
other gene names :
SHBG; SHBG; SBP; ABP; TeBG
uniprot entry name :
SHBG_HUMAN
reactivity :
Human
storage stability :
Store all reagents at 2-8 degree C.
products categories :
Signal Transduction
products description :
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This SHBG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SHBG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve.
ncbi gi num :
338075
ncbi acc num :
AAC18778.1
ncbi mol weight :
41,712 Da
size :
96 Strip Wells
price :
675 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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