ELISA/assay

Human Arginase 1 (Liver type) ELISA Kit

MBS733912

48-Strip-Wells

470 USD

ELISA Kit

Human Arginase 1 (Liver type) ELISA Kit

Arginase 1 (Liver type)

arginase-1 isoform 1; Arginase-1; arginase-1; arginase, liver; type I arginase; liver-type arginase; arginase 1; Liver-type arginase; Type I arginase

Arg1

ARG1; ARG1

ARGI1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of L-ARG1. No significant cross-reactivity or interference between L-ARG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L-ARG1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This L-ARG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human L-ARG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: L-ARG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L-ARG1 antibody and an L-ARG1-HRP conjugate. The assay sample and buffer are incubated together with L-ARG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L-ARG1 concentration since L-ARG1 from samples and L-ARG1-HRP conjugate compete for the anti-L-ARG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by L-ARG1 from the sample, fewer sites are left to bind L-ARG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-ARG1 concentration in each sample is interpolated from this standard curve.

346986435

NP_001231367.1

NM_001244438.1

25,356 Da

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolic Pathways (132956); Metabolism Pathway (477135)

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.5.3.1; Hydrolase; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus. Molecular Function: manganese ion binding; arginase activity. Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; response to amine stimulus; urea cycle; lung development. Disease: Argininemia

48-Strip-Wells

470 USD

96-Strip-Wells

675