ELISA/assay

Mouse Matrix metalloproteinase 12 ELISA Kit

MBS733903

48-Strip-Wells

470 USD

ELISA Kit

Mouse Matrix metalloproteinase 12 ELISA Kit

Matrix metalloproteinase 12

Matrix metalloproteinase 12, preproprotein; Macrophage metalloelastase; macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase); matrix metallopeptidase 12 (macrophage elastase); Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

MMP-12

MMP12; MMP12; ME; HME; MME; MMP-12; HME; MME; ME; hME; MMP-12

MMP12_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of MMP-12. No significant cross-reactivity or interference between MMP-12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-12 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MMP-12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MMP-12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: MMP-12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-12 antibody and an MMP-12-HRP conjugate. The assay sample and buffer are incubated together with MMP-12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-12 concentration since MMP-12 from samples and MMP-12-HRP conjugate compete for the anti-MMP-12 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-12 from the sample, fewer sites are left to bind MMP-12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-12 concentration in each sample is interpolated from this standard curve.

85567332

AAI12302.1

54,002 Da

Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1 site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family. Protein type: Secreted; Protease; EC 3.4.24.65; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells

48-Strip-Wells

470 USD

96-Strip-Wells

675