ELISA/assay

Human glycoalbumin ELISA Kit

MBS733902

96 Strip Wells

675 USD

ELISA Kit

Human glycoalbumin ELISA Kit

glycoalbumin

Sensitivity: The sensitivity in this assay is 1.0 ug/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of glycoalbumin. No significant cross-reactivity or interference between glycoalbumin and analogues was observed.

Store all reagents at 2-8 degree C

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This glycoalbumin ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of glycoalbumin in the sample, this glycoalbumin ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus glycoalbumin concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of glycoalbumin utilizes a multiclonal anti-glycoalbumin antibody and an glycoalbumin-HRP conjugate. The assay sample and buffer are incubated together with glycoalbumin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the glycoalbumin concentration since glycoalbumin from samples and glycoalbumin-HRP conjugate compete for the anti-glycoalbumin antibody binding site. Since the number of sites is limited, as more sites are occupied by glycoalbumin from the sample, fewer sites are left to bind glycoalbumin-HRP conjugate. Standards of known glycoalbumin concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of glycoalbumin. The glycoalbumin concentration in each sample is interpolated from this standard curve.

96 Strip Wells

675 USD