ELISA/assay

Rat Insulin like Growth Factor beta1 ELISA Kit

MBS733869

48-Strip-Wells

470 USD

ELISA Kit

Rat Insulin like Growth Factor beta1 ELISA Kit

Insulin like Growth Factor beta1

Rat Insulin like Growth Factor b1 ELISA Kit

ILGF-beta1

ILGF-b1

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This pIL-1beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat pIL-1beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: pIL-1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-pIL-1beta antibody and an pIL-1beta-HRP conjugate. The assay sample and buffer are incubated together with pIL-1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pIL-1beta concentration since pIL-1beta from samples and pIL-1beta-HRP conjugate compete for the anti-pIL-1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by pIL-1beta from the sample, fewer sites are left to bind pIL-1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pIL-1beta concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675