ELISA/assay

Sheep Aldehyde dehydrogenase family 1 member A3 ELISA Kit

MBS733865

96 Strip Wells

675 USD

ELISA Kit

Sheep Aldehyde dehydrogenase family 1 member A3 ELISA Kit

Aldehyde dehydrogenase family 1 member A3

adiponectin; Adiponectin; adiponectin; adipocyte-specific protein AdipoQ; adipocyte complement related protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ

ALDH1A3

Adipoq; Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30; Acdc; Acrp30; Apm1; ACRP30

ADIPO_MOUSE

Sheep

Store all reagents at 2-8 degree C.

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This APN ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of APN in the sample, this APN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus APN concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of APN utilizes a multiclonal anti-APN antibody and an APN-HRP conjugate. The assay sample and buffer are incubated together with APN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APN concentration since APN from samples and APN-HRP conjugate compete for the anti-APN antibody binding site. Since the number of sites is limited, as more sites are occupied by APN from the sample, fewer sites are left to bind APN-HRP conjugate. Standards of known APN concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of APN. The APN concentration in each sample is interpolated from this standard curve.

87252711

NP_033735.3

NM_009605.4

26,809 Da

AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198299!!Developmental Biology Pathway 970813!!Leptin And Adiponectin Pathway 198344!!Non-alcoholic Fatty Liver Disease (NAFLD) Pathway 862220!!Non-alcoholic Fatty Liver Disease (NAFLD) Pathway 862314!!PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway 522983

96 Strip Wells

675 USD