catalog number :
MBS733864
products type :
ELISA Kit
products full name :
Horse Oxytocin ELISA Kit
products short name :
Oxytocin
specificity :
This assay has high sensitivity and excellent specificity for detection of OXT. No significant cross-reactivity or interference between OXT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OXT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This OXT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Horse OXT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: OXT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OXT antibody and an OXT-HRP conjugate. The assay sample and buffer are incubated together with OXT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OXT concentration since OXT from samples and OXT-HRP conjugate compete for the anti-OXT antibody binding site. Since the number of sites is limited, as more sites are occupied by OXT from the sample, fewer sites are left to bind OXT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OXT concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ADK26458.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)