ELISA/assay

Cow Follicle Stimulating Hormone ELISA Kit (FSH)

MBS733860

96 Strip Wells

675 USD

ELISA Kit

Cow Follicle Stimulating Hormone ELISA Kit (FSH)

Follicle Stimulating Hormone (FSH)

follicle-stimulating hormone; Follistatin; follistatin; follistatin A; activin-binding protein; Activin-binding protein

FSH

FST; FST; FS; FS

FST_PIG

334

Sensitivity: The sensitivity in this assay is0.1 ng/ml. Specificity: This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.

Store all reagents at 2-8 degree C

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This FSH ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FSH in the sample, this FSH ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus FSH concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of FSH utilizes a multiclonal anti-FSH antibody and an FSH-HRP conjugate. The assay sample and buffer are incubated together with FSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FSH concentration since FSH from samples and FSH-HRP conjugate compete for the anti-FSH antibody binding site. Since the number of sites is limited, as more sites are occupied by FSH from the sample, fewer sites are left to bind FSH-HRP conjugate. Standards of known FSH concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of FSH. The FSH concentration in each sample is interpolated from this standard curve.

164462

AAA31038.1

P10669

38,035 Da

TGF-beta Signaling Pathway 84476!!TGF-beta Signaling Pathway 475

Function: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). Subunit structure: Monomer . Potential. Subcellular location: Secreted. Sequence similarities: Contains 3 follistatin-like domains.Contains 3 Kazal-like domains.Contains 1 TB (TGF-beta binding) domain.

96 Strip Wells

675 USD