Human Glutamate receptor 1 (GRIA1) ELISA Kit | MyBioSource MBS733849 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glutamate receptor 1 (GRIA1) ELISA Kit

catalog :

MBS733849

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733849

products type :

ELISA Kit

products full name :

Human Glutamate receptor 1 (GRIA1) ELISA Kit

products short name :

Glutamate receptor 1 (GRIA1)

other names :

glutamate receptor 1 isoform 1; Glutamate receptor 1; glutamate receptor 1; AMPA 1; gluR-1; gluR-A; gluR-K1; AMPA-selective glutamate receptor 1; glutamate receptor, ionotropic, AMPA 1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1; GluA1

other gene names :

GRIA1; GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; GLUH1; GLUR1; GluR-1; GluA1

uniprot entry name :

GRIA1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GaA. No significant cross-reactivity or interference between GaA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GaA and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GaA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GaA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: GaA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GaA antibody and an GaA-HRP conjugate. The assay sample and buffer are incubated together with GaA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GaA concentration since GaA from samples and GaA-HRP conjugate compete for the anti-GaA antibody binding site. Since the number of sites is limited, as more sites are occupied by GaA from the sample, fewer sites are left to bind GaA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GaA concentration in each sample is interpolated from this standard curve.

ncbi gi num :

167001419

ncbi acc num :

NP_000818.2

ncbi gb acc num :

NM_000827.3

ncbi mol weight :

102,864 Da

ncbi pathways :

Activation Of AMPA Receptors Pathway (106539); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); BDNF Signaling Pathway (712093); Circadian Entrainment Pathway (698773); Circadian Entrainment Pathway (699872)

ncbi summary :

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation. Protein type: Channel, calcium; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: postsynaptic membrane; recycling endosome; synaptic vesicle; endoplasmic reticulum membrane; cell surface; cell soma; postsynaptic density; dendrite; plasma membrane; dendritic spine; cell junction. Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; glutamate receptor activity; PDZ domain binding. Biological Process: synaptic transmission, glutamatergic; synaptic transmission; long-term memory; ionotropic glutamate receptor signaling pathway; receptor internalization; signal transduction

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage