ELISA/assay

Canine Parathyroid hormone ELISA Kit (PHT)

MBS733848

48-Strip-Wells

470 USD

ELISA Kit

Canine Parathyroid hormone ELISA Kit (PHT)

Parathyroid hormone ELISA Kit (PHT)

PTH

Sensitivity: The sensitivity in this assay is1.0 pg/ml. Specificity: This assay has high sensitivity and excellent specificity for detection of PHT. No significant cross-reactivity or interference between PHT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This PHT ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PHT in the sample, this PHT ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PHT concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PHT utilizes a multiclonal anti-PHT antibody and an PHT-HRP conjugate. The assay sample and buffer are incubated together with PHT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PHT concentration since PHT from samples and PHT-HRP conjugate compete for the anti-PHT antibody binding site. Since the number of sites is limited, as more sites are occupied by PHT from the sample, fewer sites are left to bind PHT-HRP conjugate. Standards of known PHT concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of PHT. The PHT concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675