ELISA/assay

Human Anti beta 1 Adrenergic Receptor antibodies ELISA Kit

MBS733845

48-Strip-Wells

470 USD

ELISA Kit

Human Anti beta 1 Adrenergic Receptor antibodies ELISA Kit

Anti beta 1 Adrenergic Receptor antibodies

Human Anti b 1 Adrenergic Receptor antibodies ELISA Kit

beta-1 adrenergic receptor; Beta-1 adrenergic receptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; adrenergic, beta-1-, receptor; adrenoceptor beta 1; Beta-1 adrenoreceptor; Beta-1 adrenoceptor

b1AR

Anti b1AR

ADRB1; ADRB1; RHR; B1AR; ADRB1R; BETA1AR; ADRB1R; B1AR; Beta-1 adrenoceptor

ADRB1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ARbeta1Ab. No significant cross-reactivity or interference between ARbeta1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARbeta1Ab and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1ng/mL.

Signal Transduction

Intended Uses This ARbeta1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARbeta1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ARbeta1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing beta 1 Adrenergic Receptor antigen and an ARbeta1Ab-HRP conjugate. The assay sample and buffer are incubated together with ARbeta1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARbeta1Ab concentration since ARbeta1Ab from samples and ARbeta1Ab-HRP conjugate compete for the beta 1 Adrenergic Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by ARbeta1Ab from the sample, fewer sites are left to bind ARbeta1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARbeta1Ab concentration in each sample is interpolated from this standard curve.

4557265

NP_000675.1

NM_000684.2

51,323 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Adrenoceptors Pathway (106365); Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285)

The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Jul 2008]

ADRB1: a GPCR-coupled 7 transmembrane receptor that binds epinephrine and norepinephrine with approximately equal affinity. Mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Interacts with PIST and PSD-95. Localized at the plasma membrane and in the Golgi upon PIST overexpression. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 10q25.3. Cellular Component: integral to plasma membrane; early endosome; plasma membrane; nucleus. Molecular Function: protein binding; protein heterodimerization activity; receptor signaling protein activity; dopamine binding; norepinephrine binding; drug binding; epinephrine binding; alpha-2A adrenergic receptor binding; beta-adrenergic receptor activity; Ras guanyl-nucleotide exchange factor activity; beta1-adrenergic receptor activity; PDZ domain binding. Biological Process: fear response; diet induced thermogenesis; glycogen catabolic process; negative regulation of multicellular organism growth; wound healing; apoptosis; positive regulation of apoptosis; adenylate cyclase activation; lipid homeostasis; positive regulation of systemic arterial blood pressure; regulation of calcium ion transport; sensory perception of pain; positive regulation of saliva secretion; memory; Rho protein signal transduction; regulation of inhibitory postsynaptic membrane potential; positive regulation of the force of heart contraction by norepinephrine; protein localization in organelle; negative regulation of smooth muscle contraction; heat generation; positive regulation of cAMP biosynthetic process; brown fat cell differentiation; response to cold; positive regulation of heart rate by epinephrine-norepinephrine; aging; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure. Disease: Resting Heart Rate, Variation In

48-Strip-Wells

470 USD

96-Strip-Wells

675