catalog number :
MBS733844
products type :
ELISA Kit
products full name :
Human Anti M2 cholinergic receptor antibodies ELISA Kit
products short name :
Anti M2 cholinergic receptor antibodies
other names :
CHRM2, partial; CHRM2; CHRM2; Uncharacterized protein
products gene name :
CHRM2
other gene names :
LOC714886
uniprot entry name :
Q8MJB0_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of AM2Ab. No significant cross-reactivity or interference between AM2Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AM2Ab and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This AM2Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AM2Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the assay: AM2Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing M2 Cholinergic Receptor antigen and an AM2Ab-HRP conjugate. The assay sample and buffer are incubated together with AM2Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AM2Ab concentration since AM2Ab from samples and AM2Ab-HRP conjugate compete for the M2 Cholinergic Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by AM2Ab from the sample, fewer sites are left to bind AM2Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AM2Ab concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAM75332.1
ncbi mol weight :
13,931 Da