ELISA/assay

Hamster Apolipoprotein B ELISA Kit (APOB)

MBS733843

48-Strip-Wells

470 USD

ELISA Kit

Hamster Apolipoprotein B ELISA Kit (APOB)

Apolipoprotein B (APOB)

apolipoprotein B; apolipoprotein B; apolipoprotein B

APOB

APOB; APOB-100; ApoB(100)

4498

This assay has high sensitivity and excellent specificity for detection of Apo-B. No significant cross-reactivity or interference between Apo-B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Apo-B and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ug/mL.

Intended Uses: This Apo-B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Hamster Apo-B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: Apo-B ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Apo-B. Standards or samples are then added to the microtiter plate wells and Apo-B if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Apo-B present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Apo-B are added to each well to "sandwich" the Apo-B immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Apo-B and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-B concentration in each sample is interpolated from this standard curve.

296482385

DAA24500.1

Cell Surface Interactions At The Vascular Wall Pathway (637405); Chylomicron-mediated Lipid Transport Pathway (637172); Fat Digestion And Absorption Pathway (194380); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (637377); LDL-mediated Lipid Transport Pathway (637174); Lipid Digestion, Mobilization, And Transport Pathway (637168); Lipoprotein Metabolism Pathway (637171); Metabolism Pathway (637141); Metabolism Of Lipids And Lipoproteins Pathway (637167)

48-Strip-Wells

470 USD

96-Strip-Wells

675