Mouse cathepsin S ELISA Kit (CTSS) | MyBioSource MBS733840 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse cathepsin S ELISA Kit (CTSS)

catalog :

MBS733840

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733840

products type :

ELISA Kit

products full name :

Mouse cathepsin S ELISA Kit (CTSS)

products short name :

Cathepsin S (CTSS)

other names :

cathepsin S; Cathepsin S; cathepsin S; cathepsin S

products gene name :

CTSS

other gene names :

CTSS; CTSS

uniprot entry name :

CATS_HUMAN

sequence length :

331

specificity :

This assay has high sensitivity and excellent specificity for detection of CTS. No significant cross-reactivity or interference between CTS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTS and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

products description :

Principle of the assay: CTS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTS antibody and an CTS-HRP conjugate. The assay sample and buffer are incubated together with CTS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTS concentration since CTS from samples and CTS-HRP conjugate compete for the anti-CTS antibody binding site. Since the number of sites is limited, as more sites are occupied by CTS from the sample, fewer sites are left to bind CTS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTS concentration in each sample is interpolated from this standard curve.

ncbi gi num :

179957

ncbi acc num :

AAC37592.1

uniprot acc num :

P25774

ncbi mol weight :

37,496 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Endosomal/Vacuolar Pathway (477125); Immune System Pathway (106386); Innate Immune System Pathway (106387); Lysosome Pathway (99052); Lysosome Pathway (96865)

ncbi summary :

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

uniprot summary :

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.22.27; Protease. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; lysosomal lumen; membrane; intracellular membrane-bound organelle; lysosome; extracellular region. Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding. Biological Process: adaptive immune response; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; antigen processing and presentation; collagen catabolic process; extracellular matrix disassembly; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage