ELISA/assay

Bovine Fetuin (FETU) ELISA Kit

MBS733819

48-Strip-Wells

470 USD

ELISA Kit

Bovine Fetuin (FETU) ELISA Kit

Fetuin (FETU)

fetuin

FETU

Bovine

345

This assay has high sensitivity and excellent specificity for detection of FETU. No significant cross-reactivity or interference between FETU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FETU and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This FETU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FETU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Bovine ELISA Kit

Principle of the assay: FETU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FETU antibody and an FETU-HRP conjugate. The assay sample and buffer are incubated together with FETU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FETU concentration since FETU from samples and FETU-HRP conjugate compete for the anti-FETU antibody binding site. Since the number of sites is limited, as more sites are occupied by FETU from the sample, fewer sites are left to bind FETU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FETU concentration in each sample is interpolated from this standard curve.

2564203

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region. Molecular Function: receptor signaling protein tyrosine kinase inhibitor activity; cysteine protease inhibitor activity. Biological Process: negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; male gonad development; regulation of inflammatory response; acute-phase response; positive regulation of bone resorption; negative regulation of insulin receptor signaling pathway; protein complex assembly; negative regulation of cell growth

48-Strip-Wells

470 USD

96-Strip-Wells

675