ELISA/assay

Human Interleukin 17E ELISA Kit

MBS733818

48-Strip-Wells

470 USD

ELISA Kit

Human Interleukin 17E ELISA Kit

Interleukin 17E

interleukin 17E; Interleukin-25; interleukin-25; interleukin-17E; interleukin 25; Interleukin-17E; IL-17E

IL 17E

IL25; IL25; IL17E; IL17E; UNQ3120/PRO10272; IL-25; IL-17E

IL25_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This IL-17E ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-17E. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY IL-17E ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-17E. Standards or samples are then added to the microtiter plate wells and IL-17E if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-17E present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-17E are added to each well to “sandwich” the IL-17E immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17E and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-17E concentration in each sample is interpolated from this standard curve.

11878210

AAG40848.1

18,523 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968)

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular space. Molecular Function: cytokine activity; interleukin-17E receptor binding. Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

470 USD

96-Strip-Wells

675