ELISA/assay

Human seminal plasma protein homolog 1 (BSPH1) ELISA Kit

MBS733816

96 Strip Wells

675 USD

ELISA Kit

Human seminal plasma protein homolog 1 (BSPH1) ELISA Kit

seminal plasma protein homolog 1 (BSPH1)

serum amyloid A-2 protein isoform b; Serum amyloid A-2 protein; serum amyloid A-2 protein; serum amyloid A2

SAA2; SAA2; SAA2

SAA2_HUMAN

Human

Store all reagents at 2-8 degree C.

Cardiovascular

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This SE ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SE in the sample, this SE ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SE concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of SE utilizes a multiclonal anti-SE antibody and an SE-HRP conjugate. The assay sample and buffer are incubated together with SE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SE concentration since SE from samples and SE-HRP conjugate compete for the anti-SE antibody binding site. Since the number of sites is limited, as more sites are occupied by SE from the sample, fewer sites are left to bind SE-HRP conjugate. Standards of known SE concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of SE. The SE concentration in each sample is interpolated from this standard curve.

188497687

NP_001120852.1

NM_001127380.2

9,184 Da

Folate Metabolism Pathway 198833!!Selenium Pathway 198825!!Vitamin B12 Metabolism Pathway 920974

96 Strip Wells

675 USD