Human Peroxiredoxin 2 ELISA Kit | MyBioSource MBS733813 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Peroxiredoxin 2 ELISA Kit

catalog :

MBS733813

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733813

products type :

ELISA Kit

products full name :

Human Peroxiredoxin 2 ELISA Kit

products short name :

Peroxiredoxin 2

other names :

Peroxiredoxin-2; peroxiredoxin-2; peroxiredoxin 2; Natural killer cell-enhancing factor B; NKEF-B; PRP; Thiol-specific antioxidant protein; TSA; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1

products gene name :

PRDX2

other gene names :

PRDX2; PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a; NKEFB; TDPX1; NKEF-B; TSA

uniprot entry name :

PRDX2_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

other info2 :

Intended Uses: This PRDX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRDX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This PRDX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRDX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the Assay: PRDX2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRDX2. Standards or samples are then added to the microtiter plate wells and PRDX2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRDX2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRDX2 are added to each well to "sandwich" the PRDX2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRDX2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRDX2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

33188454

ncbi acc num :

NP_859428.1

ncbi gb acc num :

NM_181738.1

ncbi mol weight :

15,819 Da

ncbi pathways :

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Selenium Pathway (198825)

ncbi summary :

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013]

uniprot summary :

PRDX2: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression. Three splice-variant isoforms have been identified. Protein type: Oxidoreductase; EC 1.11.1.15. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: cytoplasm; cytosol. Molecular Function: antioxidant activity; thioredoxin peroxidase activity. Biological Process: regulation of apoptosis; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of apoptosis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage