Human C4 Binding Protein, Alpha ELISA Kit | MyBioSource MBS733812 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human C4 Binding Protein, Alpha ELISA Kit

catalog :

MBS733812

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733812

products type :

ELISA Kit

products full name :

Human C4 Binding Protein, Alpha ELISA Kit

products short name :

C4 Binding Protein, Alpha

other names :

C4b-binding protein alpha chain; C4b-binding protein alpha chain; C4b-binding protein alpha chain; proline-rich protein; complement component 4 binding protein, alpha; Proline-rich protein; PRP

products gene name :

C4BPa

other gene names :

C4BPA; C4BPA; PRP; C4BP; C4BP; C4bp; PRP

uniprot entry name :

C4BPA_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

products categories :

Immunology

products description :

Intended Uses: This C4BPa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human C4BPa. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: C4BPa ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C4BPa. Standards or samples are then added to the microtiter plate wells and C4BPa if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C4BPa present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C4BPa are added to each well to "sandwich" the C4BPa immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C4BPa and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4BPa concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4502503

ncbi acc num :

NP_000706.1

ncbi gb acc num :

NM_000715.3

ncbi mol weight :

67,033 Da

ncbi pathways :

CD40/CD40L Signaling Pathway (138061); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Innate Immune System Pathway (106387); Pertussis Pathway (218111); Pertussis Pathway (218099); Regulation Of Complement Cascade Pathway (576254)

ncbi summary :

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

uniprot summary :

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; extracellular region; plasma membrane. Molecular Function: protein binding. Biological Process: negative regulation of complement activation, classical pathway; regulation of complement activation; positive regulation of protein catabolic process; innate immune response; complement activation, classical pathway

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage