ELISA/assay

Human Bruton's Tyrosine Kinase ELISA Kit

MBS733788

48-Strip-Wells

470 USD

ELISA Kit

Human Bruton's Tyrosine Kinase ELISA Kit

Bruton's Tyrosine Kinase

Bruton's tyrosine kinase; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; truncated Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; Bruton agammaglobulinemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase

Btk

BTK; BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1; AGMX1; ATK; BPK; ATK; BPK

BTK_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This BTK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BTK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BTK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BTK. Standards or samples are then added to the microtiter plate wells and BTK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BTK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BTK are added to each well to "sandwich" the BTK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BTK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTK concentration in each sample is interpolated from this standard curve.

1684918

AAB64205.1

79,937 Da

Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Class I PI3K Signaling Events Pathway (138022); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis. Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Tec family. Chromosomal Location of Human Ortholog: Xq21.33-q22. Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft. Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding. Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; B cell activation; transcription, DNA-dependent; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of cell proliferation; toll-like receptor 2 signaling pathway; regulation of B cell apoptosis; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked

48-Strip-Wells

470 USD

96-Strip-Wells

675