product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human 1, 25 dihydroxyvitamin D3 ELISA Kit
catalog :
MBS733782
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS733782
products type :
ELISA Kit
products full name :
Human 1, 25 dihydroxyvitamin D3 ELISA Kit
products short name :
1, 25 dihydroxyvitamin D3
products gene name :
1, 25 (OH) 2D3
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of 1,25(OH)2D. No significant cross-reactivity or interference between 1,25(OH)2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 1,25(OH)2D and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products description :
Intended Uses: This 1,25(OH)2D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 1,25(OH)2D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay 1,25(OH)2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-1,25(OH)2D antibody and an 1,25(OH)2D-HRP conjugate. The assay sample and buffer are incubated together with 1,25(OH)2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25(OH)2D concentration since 1,25(OH)2D from samples and 1,25(OH)2D-HRP conjugate compete for the anti-1,25(OH)2D antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25(OH)2D from the sample, fewer sites are left to bind 1,25(OH)2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25(OH)2D concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!