Human Ceruloplasmin, CP/CER ELISA Kit | MyBioSource MBS733748 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ceruloplasmin, CP/CER ELISA Kit

catalog :

MBS733748

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733748

products type :

ELISA Kit

products full name :

Human Ceruloplasmin, CP/CER ELISA Kit

products short name :

Ceruloplasmin, CP/CER

products name syn :

Human Ceruloplasmin,CP/CER ELISA Kit (CP/CER) ELISA Kit; Human Ceruloplasmin, CP/CER ELISA Kit; Ceruloplasmin, CP/CER; Ceruloplasmin, CP/CER ELISA Kit (Human)

other names :

ceruloplasmin; Ceruloplasmin; ceruloplasmin; ceruloplasmin (ferroxidase); Ferroxidase

products gene name :

CP/CER

other gene names :

CP; CP; CP-2

uniprot entry name :

CERU_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CER. No significant cross-reactivity or interference between CER and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CER and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This CER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CER. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Human ELISA Kit

products description :

Principle of the Assay: CER ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CER. Standards or samples are then added to the microtiter plate wells and CER if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CER present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CER are added to each well to "sandwich" the CER immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CER and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CER concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4557485

ncbi acc num :

NP_000087.1

ncbi gb acc num :

NM_000096.3

uniprot acc num :

P00450

ncbi mol weight :

122,205 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

ncbi summary :

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

uniprot summary :

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family. Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q23-q25. Cellular Component: extracellular space; lysosomal membrane; extracellular region. Molecular Function: ferroxidase activity; copper ion binding; chaperone binding. Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport. Disease: Aceruloplasminemia

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage