ELISA/assay

Human Vinculin ELISA Kit

MBS733711

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Vinculin ELISA Kit

Vinculin

vinculin, partial; Vinculin; vinculin; metavinculin; epididymis luminal protein 114; vinculin; Metavinculin; MV

VCL

VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV

VINC_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of VCL. No significant cross-reactivity or interference between VCL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VCL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL

Signal Transduction

Intended Uses: This VCL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VCL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VCL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VCL. Standards or samples are then added to the microtiter plate wells and VCL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VCL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VCL are added to each well to "sandwich" the VCL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VCL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCL concentration in each sample is interpolated from this standard curve.

246714

AAB21657.1

24,904 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: protein complex; costamere; focal adhesion; extracellular region; intercellular junction; fascia adherens; cytosol; actin cytoskeleton; cell-matrix junction; cytoskeleton; adherens junction; cell-cell adherens junction; plasma membrane; vesicle. Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding. Biological Process: lamellipodium biogenesis; platelet activation; apical junction assembly; platelet degranulation; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; blood coagulation; cell motility; negative regulation of cell migration. Disease: Cardiomyopathy, Dilated, 1w

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675