catalog number :
MBS733705
products type :
ELISA Kit
products full name :
Mouse Growth Differentiation Factor 9 ELISA Kit
products short name :
Growth Differentiation Factor 9
other names :
growth differentiation factor 9; Growth differentiation factor 9; growth differentiation factor 9; growth/differentiation factor 9; Growth differentiation factor 9; Uncharacterized protein
products gene name :
GDF9
other gene names :
GDF9; GDF9
uniprot entry name :
C5HV43_AILME
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products categories :
Cancer
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This GDF-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GDF-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY GDF-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-9 antibody and an GDF-9-HRP conjugate. The assay sample and buffer are incubated together with GDF-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-9 concentration since GDF-9 from samples and GDF-9-HRP conjugate compete for the anti-GDF-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-9 from the sample, fewer sites are left to bind GDF-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-9 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ACR48174.1
ncbi mol weight :
51,629 Da
ncbi pathways :
Ovarian Steroidogenesis Pathway (790082); Ovarian Steroidogenesis Pathway (795173)