Rat Cathelicidin Antimicrobial Peptide ELISA Kit | MyBioSource MBS733693 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Cathelicidin Antimicrobial Peptide ELISA Kit

catalog :

MBS733693

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733693

products type :

ELISA Kit

products full name :

Rat Cathelicidin Antimicrobial Peptide ELISA Kit

products short name :

Cathelicidin Antimicrobial Peptide

other names :

CAMP, partial; Cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein; CAP-18; hCAP-18

products gene name :

CAMP

other gene names :

CAMP; CAP18; FALL39; HSD26; CAP-18; hCAP-18

uniprot entry name :

CAMP_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of CAMP. No significant cross-reactivity or interference between CAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

products categories :

Microbiology

products description :

Intended Uses: This CAMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CAMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CAMP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CAMP. Standards or samples are then added to the microtiter plate wells and CAMP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CAMP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CAMP are added to each well to "sandwich" the CAMP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CAMP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

49456877

ncbi acc num :

CAG46759.1

uniprot acc num :

P49913

ncbi mol weight :

19,301 Da

ncbi pathways :

Disease Pathway (530764); Integrated Pancreatic Cancer Pathway (711360); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); SREBP Signalling Pathway (219802); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]

uniprot summary :

CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family. Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation; Vesicle. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol. Molecular Function: protein binding. Biological Process: interaction with host; positive regulation of angiogenesis; defense response to Gram-positive bacterium; innate immune response in mucosa; killing by host of symbiont cells; defense response to bacterium; positive regulation of cell proliferation; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage