Rabbit Defensin 1, Neutrophil ELISA Kit | MyBioSource MBS733632 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Defensin 1, Neutrophil ELISA Kit

catalog :

MBS733632

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733632

products type :

ELISA Kit

products full name :

Rabbit Defensin 1, Neutrophil ELISA Kit

products short name :

Defensin 1, Neutrophil

other names :

defensin, alpha 1, myeloid-related sequence; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1; Defensin, alpha 1; HNP-1; HP-1; HP1

products gene name :

DEFA1

other gene names :

DEFA1; DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1; DEF1; DEFA2; MRS DEFA1B ; HP-1; HP1; HP-2; HP2

uniprot entry name :

DEF1_HUMAN

reactivity :

Rabbit

specificity :

This assay has high sensitivity and excellent specificity for detection of DEF1. No significant cross-reactivity or interference between DEF1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DEF1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This DEF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit DEF1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: DEF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEF1 antibody and an DEF1-HRP conjugate. The assay sample and buffer are incubated together with DEF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEF1 concentration since DEF1 from samples and DEF1-HRP conjugate compete for the anti-DEF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEF1 from the sample, fewer sites are left to bind DEF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEF1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

50057842

ncbi acc num :

AAT68878.1

ncbi mol weight :

10,201 Da

ncbi pathways :

Alpha-defensins Pathway (530758); Cellular Roles Of Anthrax Toxin Pathway (138076); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

uniprot summary :

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region. Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to fungus; defense response to virus

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage