ELISA/assay

Human G Protein Coupled Receptor ELISA Kit

MBS733550

96 Strip Wells

675 USD

ELISA Kit

Human G Protein Coupled Receptor ELISA Kit

G Protein Coupled Receptor

G-protein coupled receptor 2; LanC-like protein GCR2; G-protein coupled receptor 2; G-protein coupled receptor 2

GPCR

GPCR; GCR2; F14G24.19; F14G24_19; G protein coupled receptor; G-PROTEIN COUPLED RECEPTOR 2; GCR2; GPCR; GPCR; At1g52920; F14G24.19

GCR2_ARATH

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This GPCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GPCR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sentivity in this assay is 0.1 ng/mL.

Signal Transduction

Principle of the Assay: GPCR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GPCR. Standards or samples are then added to the microtiter plate wells and GPCR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GPCR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GPCR are added to each well to "sandwich" the GPCR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GPCR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPCR concentration in each sample is interpolated from this standard curve.

145336679

NP_175700.2

NM_104170.2

46,451 Da

Encodes a plasma membrane localized ABA receptor, which interacts with the G complex. It has been postulated that the binding of ABA to GCR2 results in the release of the G protein and dissociation of the heterotrimeric complex into G and the G dimer to activate downstream ABA effectors and to trigger the ABA responses.

96 Strip Wells

675 USD