Human Prostaglandin Endoperoxide Synthase 1 ELISA Kit | MyBioSource MBS733514 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Prostaglandin Endoperoxide Synthase 1 ELISA Kit

catalog :

MBS733514

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733514

products type :

ELISA Kit

products full name :

Human Prostaglandin Endoperoxide Synthase 1 ELISA Kit

products short name :

Prostaglandin Endoperoxide Synthase 1

other names :

prostaglandin-endoperoxide synthase 1; Prostaglandin G/H synthase 1; prostaglandin G/H synthase 1; PGH synthase 1; cyclooxygenase-1; prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1

products gene name :

PTGS1

other gene names :

PTGS1; PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1; COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1

uniprot entry name :

PGH1_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This PTGS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTGS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: PTGS1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PTGS1. Standards or samples are then added to the microtiter plate wells and PTGS1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PTGS1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PTGS1 are added to each well to "sandwich" the PTGS1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PTGS1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGS1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

17017287

ncbi acc num :

AAL33601.1

ncbi mol weight :

72,010 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); C20 Prostanoid Biosynthesis Pathway (908107); COX Reactions Pathway (105711); Eicosanoid Synthesis Pathway (198888); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

COX-1: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.14.99.1; Lipid Metabolism - arachidonic acid; Oxidoreductase. Chromosomal Location of Human Ortholog: 9q32-q33.3. Cellular Component: endoplasmic reticulum membrane; photoreceptor outer segment; intracellular membrane-bound organelle; cytoplasm; nucleus. Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; dioxygenase activity; metal ion binding; heme binding. Biological Process: regulation of blood pressure; xenobiotic metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; response to oxidative stress; lipid metabolic process; prostaglandin biosynthetic process; inflammatory response; regulation of cell proliferation

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage