ELISA/assay

Mouse Fibromodulin ELISA Kit

MBS733452

48-Strip-Wells

440 USD

ELISA Kit

Mouse Fibromodulin ELISA Kit

[Fibromodulin]

[fibromodulin; Fibromodulin; fibromodulin; KSPG fibromodulin; collagen-binding 59 kDa protein; keratan sulfate proteoglycan fibromodulin; fibromodulin; Collagen-binding 59 kDa protein; Keratan sulfate proteoglycan fibromodulin; KSPG fibromodulin]

[FMOD]

[FMOD; FMOD; FM; SLRR2E; FM; SLRR2E; FM; KSPG fibromodulin]

FMOD_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FMOD. No significant cross-reactivity or interference between FMOD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FMOD and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 1.0-25ng/mL. Sensitivity: 0.1ng/mL

Cardiovascular

Intended Uses: This FMOD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FMOD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FMOD ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FMOD. Standards or samples are then added to the microtiter plate wells and FMOD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FMOD present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FMOD are added to each well to "sandwich" the FMOD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FMOD and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FMOD concentration in each sample is interpolated from this standard curve.

71040111

NP_002014.2

NM_002023.4

43,179 Da

Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538)

Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

FMOD: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; extracellular region. Biological Process: transforming growth factor beta receptor complex assembly; keratan sulfate metabolic process; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; keratan sulfate catabolic process

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415