catalog number :
MBS733447
products type :
ELISA Kit
products full name :
Mouse Histone H2A-H2B-DNA autoantibody ELISA Kit
products short name :
Histone H2A-H2B-DNA autoantibody
other names :
Hha; Gene expression modulator; Gene expression modulator; Hha
other gene names :
hha; CY43_02700
uniprot entry name :
H9L4I9_SALTM
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This HHA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HHA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cell Biology
products description :
Principle of the Assay: HHA ELISA kit applies the competitive enzyme immunoassay technique utilizing Histone H2A-H2B-DNA antigen and an HHA-HRP conjugate. The assay sample and buffer are incubated together with HHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HHA concentration since HHA from samples and HHA-HRP conjugate compete for the Histone H2A-H2B-DNA antigen binding site. Since the number of sites is limited, as more sites are occupied by HHA from the sample, fewer sites are left to bind HHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HHA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAF67181.1
ncbi mol weight :
8,614 Da