ELISA/assay

Rabbit Complement Component 3a Receptor 1 ELISA Kit

MBS733432

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Complement Component 3a Receptor 1 ELISA Kit

Complement Component 3a Receptor 1

C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1; complement component 3a receptor 1

C3aR1

C3AR1; C3AR1; AZ3B; C3AR; HNFAG09; AZ3B; C3R1; HNFAG09; C3AR; C3a-R

C3AR_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ?g/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. INTENDED USE This C3aR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit C3aR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY C3aR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C3aR1. Standards or samples are then added to the microtiter plate wells and C3aR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C3aR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C3aR1 are added to each well to “sandwich” the C3aR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C3aR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3aR1 concentration in each sample is interpolated from this standard curve.

4757888

NP_004045.1

NM_004054.2

53,864 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; blood circulation; metabolic process; complement receptor mediated signaling pathway; chemotaxis; inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675