Human Pulmonary Surfactant Associated Protein C ELISA Kit | MyBioSource MBS733281 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Pulmonary Surfactant Associated Protein C ELISA Kit

catalog :

MBS733281

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733281

products type :

ELISA Kit

products full name :

Human Pulmonary Surfactant Associated Protein C ELISA Kit

products short name :

Pulmonary Surfactant Associated Protein C

other names :

pulmonary surfactant-associated protein C; Pulmonary surfactant-associated protein C; pulmonary surfactant-associated protein C; pulmonary surfactant-associated proteolipid SPL(Val); surfactant associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5

products gene name :

SPC

other gene names :

Sftpc; Sftpc; SP5; SPC; SP-C; Sftp2; Bricd6; Sftp-2; pro-SpC; Sftp2; SP-C

uniprot entry name :

PSPC_MOUSE

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of SPC. No significant cross-reactivity or interference between SPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPC and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This SPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SPC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: SPC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPC. Standards or samples are then added to the microtiter plate wells and SPC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SPC are added to each well to "sandwich" the SPC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPC concentration in each sample is interpolated from this standard curve.

ncbi gi num :

256355065

ncbi acc num :

NP_035489.2

ncbi gb acc num :

NM_011359.2

ncbi mol weight :

21,055 Da

uniprot summary :

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called hyaline membranes . 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid-binding. Cellular Component: multivesicular body; extracellular space; cytoplasm; extracellular region. Molecular Function: protein homodimerization activity. Biological Process: protein homooligomerization

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage