ELISA/assay

Human Cardiotrophin Like Cytokine Factor 1 ELISA Kit

MBS733204

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Cardiotrophin Like Cytokine Factor 1 ELISA Kit

Cardiotrophin Like Cytokine Factor 1

cardiotrophin-like cytokine factor 1 isoform 2; Cardiotrophin-like cytokine factor 1; cardiotrophin-like cytokine factor 1; novel neurotrophin-1; B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; cardiotrophin-like cytokine factor 1; B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1

CLCF1

CLCF1; CLCF1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1; BSF3; CLC; NNT1; BSF-3; NNT-1

CLCF1_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Highest Standards: 2000pg/mL. Concentration of Standards: Concentration of Standards: 0,100,250,500,1000,2500pg/mL

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This CLCF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLCF1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY CLCF1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CLCF1. Standards or samples are then added to the microtiter plate wells and CLCF1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CLCF1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CLCF1 are added to each well to “sandwich” the CLCF1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CLCF1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLCF1 concentration in each sample is interpolated from this standard curve.

261245022

NP_001159684.1

NM_001166212.1

24,087 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q13.3. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding. Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; B cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of astrocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein. Disease: Cold-induced Sweating Syndrome 2

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675