ELISA/assay

Human Hyaluronate binding protein ELISA Kit

MBS733122

48-Strip-Wells

470 USD

ELISA Kit

Human Hyaluronate binding protein ELISA Kit

Hyaluronate binding protein

Human Hyaluronate binding protein ELISA Kit; Hyaluronate binding protein; Hyaluronate binding protein (Human)

tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-inducible gene 6 protein; TNF alpha-induced protein 6; hyaluronate-binding protein; TNF-stimulated gene 6 protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein; tumor necrosis factor, alpha-induced protein 6; Hyaluronate-binding protein; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6

HABP

TNFAIP6; TNFAIP6; TSG6; TSG-6; TSG6; TSG-6

TSG6_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of HABP. No significant cross-reactivity or interference between HABP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HABP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

Human ELISA Kit

Intended Uses: This HABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HABP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HABP antibody and an HABP-HRP conjugate. The assay sample and buffer are incubated together with HABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HABP concentration since HABP from samples and HABP-HRP conjugate compete for the anti-HABP antibody binding site. Since the number of sites is limited, as more sites are occupied by HABP from the sample, fewer sites are left to bind HABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HABP concentration in each sample is interpolated from this standard curve.

26051243

NP_009046.2

NM_007115.3

P98066

31,203 Da

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]

TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis. Chromosomal Location of Human Ortholog: 2q23.3. Cellular Component: extracellular space. Molecular Function: hyaluronic acid binding. Biological Process: ovulation; cell-cell signaling; inflammatory response; cell adhesion; signal transduction; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675