Mouse Apolipoprotein A4 ELISA Kit | MyBioSource MBS733109 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein A4 ELISA Kit

catalog :

MBS733109

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS733109

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein A4 ELISA Kit

products short name :

Apolipoprotein A4

other names :

apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV; Apolipoprotein A4

products gene name :

APOA4

other gene names :

APOA4; APOA4; Apo-AIV; ApoA-IV

uniprot entry name :

APOA4_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of Apo-A4. No significant cross-reactivity or interference between Apo-A4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Apo-A4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This Apo-A4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Apo-A4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the assay: Apo-A4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Apo-A4 antibody and an Apo-A4-HRP conjugate. The assay sample and buffer are incubated together with Apo-A4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo-A4 concentration since Apo-A4 from samples and Apo-A4-HRP conjugate compete for the anti-Apo-A4 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo-A4 from the sample, fewer sites are left to bind Apo-A4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-A4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

71773110

ncbi acc num :

NP_000473.2

ncbi gb acc num :

NM_000482.3

ncbi mol weight :

45,399 Da

ncbi pathways :

Amyloids Pathway (366238); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

uniprot summary :

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Lipid-binding; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: lipid transporter activity; antioxidant activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding. Biological Process: cholesterol metabolic process; multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; phototransduction, visible light; removal of superoxide radicals; neurite regeneration; protein-lipid complex assembly; lipid homeostasis; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; phospholipid efflux; regulation of cholesterol absorption; innate immune response in mucosa; hydrogen peroxide catabolic process; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport; retinoid metabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage