ELISA/assay

Human growth hormone releasing peptide-Ghrelin ELISA Kit

MBS733049

48-Strip-Wells

470 USD

ELISA Kit

Human growth hormone releasing peptide-Ghrelin ELISA Kit

growth hormone releasing peptide-Ghrelin

Human growth hormone releasing peptide-Ghrelin ELISA Kit; growth hormone releasing peptide-Ghrelin; growth hormone releasing peptide-Ghrelin (Human)

ghrelin

GHRP-Ghrelin

Human

This assay has high sensitivity and excellent specificity for detection of GHRP-Ghrelin. No significant cross-reactivity or interference between GHRP-Ghrelin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GHRP-Ghrelin and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GHRP-Ghrelin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GHRP-Ghrelin. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Human ELISA Kit

Principle of the assay: GHRP-Ghrelin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GHRP-Ghrelin antibody and an GHRP-Ghrelin-HRP conjugate. The assay sample and buffer are incubated together with GHRP-Ghrelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHRP-Ghrelin concentration since GHRP-Ghrelin from samples and GHRP-Ghrelin-HRP conjugate compete for the anti-GHRP-Ghrelin antibody binding site. Since the number of sites is limited, as more sites are occupied by GHRP-Ghrelin from the sample, fewer sites are left to bind GHRP-Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHRP-Ghrelin concentration in each sample is interpolated from this standard curve.

304435649

ADM33790

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach. Protein type: Secreted; Apoptosis; Cell development/differentiation; Secreted, signal peptide; Cell cycle regulation. Chromosomal Location of Human Ortholog: 3p26-p25. Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity. Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of multicellular organism growth; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis. Disease: Obesity

48-Strip-Wells

470 USD

96-Strip-Wells

675