ELISA/assay

Mouse amyloid beta peptide 1-42 ELISA Kit

MBS733037

48-Strip-Wells

470 USD

ELISA Kit

Mouse amyloid beta peptide 1-42 ELISA Kit

amyloid beta peptide 1-42

Mouse amyloid beta peptide 1-42 ELISA Kit; amyloid beta peptide 1-42; amyloid beta peptide 1-42 (Mouse)

amyloid beta A4 protein isoform 6; Amyloid beta A4 protein; amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog; amyloid beta (A4) precursor protein; ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99; Alternative name(s):; APP-C99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; APP-C59; Amyloid intracellular domain 59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; APP-C57; Amyloid intracellular domain 57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AID(50); Gamma-CTF(50)

Abeta1-42

Ab1-42; A 1-42

App; App; Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik; APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50)

A4_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of betaAP1-42. No significant cross-reactivity or interference between betaAP1-42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between betaAP1-42 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Mouse ELISA Kit

Intended Uses: This betaAP1-42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse betaAP1-42. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: AP1-42 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for betaAP1-42. Standards or samples are then added to the microtiter plate wells and betaAP1-42 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of betaAP1-42 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for betaAP1-42 are added to each well to "sandwich" the betaAP1-42 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain betaAP1-42 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaAP1-42 concentration in each sample is interpolated from this standard curve.

311893408

NP_001185755.1

NM_001198826.1

P12023

86,722 Da

Activated TLR4 Signalling Pathway (819629); Advanced Glycosylation Endproduct Receptor Signaling Pathway (819650); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amyloids Pathway (819491); Class A/1 (Rhodopsin-like Receptors) Pathway (819840); Cytosolic Sensors Of Pathogen-associated DNA Pathway (819660); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (819668); Delta-Notch Signaling Pathway (198316); Disease Pathway (819462)

APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer s disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family. Protein type: Cell surface; Receptor, misc.; Apoptosis; Transcription factor; Membrane protein, integral. Cellular Component: Golgi apparatus; extracellular space; cell surface; smooth endoplasmic reticulum; integral to membrane; terminal button; coated pit; intercellular junction; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; cytoplasm; cytoplasmic vesicle; intracellular; neuromuscular junction; endosome; receptor complex; neuron projection; intracellular membrane-bound organelle; cell; apical part of cell; axon; plasma membrane; spindle midzone. Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; identical protein binding; protein binding; protease activator activity; enzyme binding; DNA binding; transition metal ion binding; protease inhibitor activity; metal ion binding; PTB domain binding; acetylcholine receptor binding; receptor binding. Biological Process: extracellular matrix organization and biogenesis; apoptosis; adult locomotory behavior; negative regulation of peptidase activity; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; visual learning; collateral sprouting in the absence of injury; neuromuscular process controlling balance; cell adhesion; neurite development; protein homooligomerization; nervous system development; cholesterol metabolic process; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; mating behavior; axon cargo transport; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; negative regulation of neuron differentiation; axonogenesis; suckling behavior; regulation of gene expression; ionotropic glutamate receptor signaling pathway; regulation of synapse structure and activity; regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; neuron remodeling

48-Strip-Wells

470 USD

96-Strip-Wells

675