ELISA/assay

Rat Stathmin 1 ELISA Kit

MBS733001

48-Strip-Wells

470 USD

ELISA Kit

Rat Stathmin 1 ELISA Kit

Stathmin 1

stathmin 1, partial; Stathmin; stathmin; prosolin; metablastin; oncoprotein 18; phosphoprotein 19; phosphoprotein p19; stathmin 1/oncoprotein 18; transmembrane protein C1orf215; leukemia-associated phosphoprotein p18; stathmin 1; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17

STMN1

STMN1; STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215; C1orf215; LAP18; OP18; Op18; pp19

STMN1_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

Intended Uses: This STMN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This STMN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STMN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: STMN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for STMN1. Standards or samples are then added to the microtiter plate wells and STMN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STMN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for STMN1 are added to each well to "sandwich" the STMN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STMN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STMN1 concentration in each sample is interpolated from this standard curve.

197692597

BAG70262.1

19,824 Da

Aurora B Signaling Pathway (138080); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); RB In Cancer Pathway (920977); Regulation Of Microtubule Cytoskeleton Pathway (755439); Signaling Mediated By P38-gamma And P38-delta Pathway (138043)

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: microtubule; neuron projection; membrane; cytoplasm; intracellular; cytosol. Molecular Function: tubulin binding; signal transducer activity. Biological Process: mitotic spindle organization and biogenesis; axonogenesis; negative regulation of microtubule polymerization; response to virus; microtubule depolymerization; positive regulation of cell motility; brain development; signal transduction; neurite development; regulation of cytoskeleton organization and biogenesis

48-Strip-Wells

470 USD

96-Strip-Wells

675