ELISA/assay

Human platelet-derived growth factor-BB ELISA Kit

MBS732941

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human platelet-derived growth factor-BB ELISA Kit

platelet-derived growth factor-BB

platelet-derived growth factor-BB; Platelet-derived growth factor-BB

PDGF-BB

A2NWD3_HUMAN

Human

161

This assay has high sensitivity and excellent specificity for detection of PDGF-BB. No significant cross-reactivity or interference between PDGF-BB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDGF-BB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This PDGF-BB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDGF-BB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

. Principle of the assay: PDGF-BB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDGF-BB antibody and an PDGF-BB-HRP conjugate. The assay sample and buffer are incubated together with PDGF-BB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGF-BB concentration since PDGF-BB from samples and PDGF-BB-HRP conjugate compete for the anti-PDGF-BB antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGF-BB from the sample, fewer sites are left to bind PDGF-BB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGF-BB concentration in each sample is interpolated from this standard curve.

35377

CAA45383.1

A2NWD3

18,237 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Sequence similarities: Belongs to the PDGF/VEGF growth factor family. RuleBase RU003818

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675