Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit | MyBioSource MBS732915 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit

catalog :

MBS732915

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732915

products type :

ELISA Kit

products full name :

Human Anti Glutamic Acid Decarboxylase Antibodies ELISA Kit

products short name :

Anti Glutamic Acid Decarboxylase Antibodies

other names :

GAD; Glutamate decarboxylase 1; glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform; glutamate decarboxylase 1 (brain, 67kDa); 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform

products gene name :

GAD

other gene names :

GAD1; GAD1; GAD; SCP; CPSQ1; GAD; GAD67; GAD-67

uniprot entry name :

DCE1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of Anti-GAD. No significant cross-reactivity or interference between Anti-GAD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-GAD and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

products categories :

Immunology

products description :

Intended Uses: This Anti-GAD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-GAD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Anti-GAD LISA kit applies the competitive enzyme immunoassay technique utilizing Glutamic Acid Decarboxylase antigen and an Anti-GAD-HRP conjugate. The assay sample and buffer are incubated together with Anti-GAD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GAD concentration since Anti-GAD from samples and Anti-GAD-HRP conjugate compete for the Glutamic Acid Decarboxylase antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-GAD from the sample, fewer sites are left to bind Anti-GAD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GAD concentration in each sample is interpolated from this standard curve.

ncbi gi num :

1247492

ncbi acc num :

CAA01913.1

ncbi mol weight :

47,440 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

uniprot summary :

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Mitochondrial. Chromosomal Location of Human Ortholog: 2q31. Cellular Component: plasma membrane; intracellular; vesicle membrane. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding. Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process. Disease: Cerebral Palsy, Spastic Quadriplegic, 1

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage