ELISA/assay

Human Z DNA binding protein ELISA Kit

MBS732908

96 Strip Wells

675 USD

ELISA Kit

Human Z DNA binding protein ELISA Kit

Z DNA binding protein

Z-DNA-binding protein 1 isoform d; Z-DNA-binding protein 1; Z-DNA-binding protein 1; DNA-dependent activator of IRFs; DNA-dependent activator of IFN-regulatory factors; tumor stroma and activated macrophage protein DLM-1; DNA-dependent activator of interferon regulatory factors; Z-DNA binding protein 1; Tumor stroma and activated macrophage protein DLM-1

ZBP1

ZBP1; ZBP1; DAI; DLM1; DLM-1; C20orf183; C20orf183; DLM1

ZBP1_HUMAN

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

ELISA Method: Sandwich. Highest Standards: 5000pg/mL. Concentration of Standards: Concentration of Standards: 0,250,500,1000,2500,5000pg/mL

Cell Biology

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . Intended Uses: This ZBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . Principle of the Assay: ZBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZBP1 antibody and an ZBP1-HRP conjugate. The assay sample and buffer are incubated together with ZBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBP1 concentration since ZBP1 from samples and ZBP1-HRP conjugate compete for the anti-ZBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBP1 from the sample, fewer sites are left to bind ZBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBP1 concentration in each sample is interpolated from this standard curve.

238550212

NP_001153891.1

NM_001160419.2

38,220 Da

Cytosolic DNA-sensing Pathway 125137!!Cytosolic DNA-sensing Pathway 124833!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!IRF3 Mediated Activation Of Type 1 IFN Pathway 576257!!IRF3-mediated Induction Of Type I IFN Pathway 833819!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!RIP-mediated NFkB Activation Via ZBP1 Pathway 576258!!Regulation Of Innate Immune Responses To Cytosolic DNA Pathway 833823!!STING Mediated Induction Of Host Immune Responses Pathway 685548

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

96 Strip Wells

675 USD