ELISA/assay

Human Beclin 1 ELISA Kit

MBS732891

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Beclin 1 ELISA Kit

Beclin 1

beclin 1; Beclin-1; beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197

Beclin 1

BECN1; BECN1; ATG6; VPS30; beclin1; GT197

BECN1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of BECN-1. No significant cross-reactivity or interference between BECN-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BECN-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Cancer

Intended Uses: This BECN-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BECN-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay BECN-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BECN-1. Standards or samples are then added to the microtiter plate wells and BECN-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BECN-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BECN-1 are added to each well to "sandwich" the BECN-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BECN-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BECN-1 concentration in each sample is interpolated from this standard curve.

4680381

AAD27650.1

51,896 Da

Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Protein type: Autophagy; Membrane protein, peripheral; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; phagocytic vesicle; trans-Golgi network; nucleus; endosome. Molecular Function: protein binding; phosphoinositide 3-kinase binding. Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; regulation of catalytic activity; lysosome organization and biogenesis; beta-amyloid metabolic process; response to hypoxia; mitochondrion degradation; neuron development; cellular defense response; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; autophagic vacuole formation; negative regulation of apoptosis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675