ELISA/assay

Human Apoptosis-related genes ELISA Kit

MBS732848

48-Strip-Wells

470 USD

ELISA Kit

Human Apoptosis-related genes ELISA Kit

Apoptosis-related genes

Human Apoptosis-related genes ELISA Kit; Apoptosis-related genes; Apoptosis-related genes ELISA Kit (Human)

apoptosis-related protein, partial

Human

This assay has high sensitivity and excellent specificity for detection of ARGs. No significant cross-reactivity or interference between ARGs and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARGs and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ARGs ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARGs. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Human ELISA Kit

Priciple of the assay: ARGs ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARGs antibody and an ARGs-HRP conjugate. The assay sample and buffer are incubated together with ARGs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARGs concentration since ARGs from samples and ARGs-HRP conjugate compete for the anti-ARGs antibody binding site. Since the number of sites is limited, as more sites are occupied by ARGs from the sample, fewer sites are left to bind ARGs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARGs concentration in each sample is interpolated from this standard curve.

45269013

AAS55919.1

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

48-Strip-Wells

470 USD

96-Strip-Wells

675