ELISA/assay

Rat Chemokine C-X3-C-Motif Receptor 1 ELISA Kit

MBS732818

48-Strip-Wells

470 USD

ELISA Kit

Rat Chemokine C-X3-C-Motif Receptor 1 ELISA Kit

Chemokine C-X3-C-Motif Receptor 1

CX3C chemokine receptor 1; CX3C chemokine receptor 1; CX3C chemokine receptor 1; CMK-BRL1; CMK-BRL-1; C-X3-C CKR-1; fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; chemokine (C-X3-C) receptor 1; beta chemokine receptor-like 1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13; V28

CX3CR1

CX3CR1; CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1; CMKBRL1; GPR13; C-X3-C CKR-1; CX3CR1; CMK-BRL1

CX3C1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CX3CR1. No significant cross-reactivity or interference between CX3CR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CX3CR1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This CX3CR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CX3CR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CX3CR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX3CR1 antibody and an CX3CR1-HRP conjugate. The assay sample and buffer are incubated together with CX3CR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX3CR1 concentration since CX3CR1 from samples and CX3CR1-HRP conjugate compete for the anti-CX3CR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX3CR1 from the sample, fewer sites are left to bind CX3CR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3CR1 concentration in each sample is interpolated from this standard curve.

1351394

P49238.1

43,969 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Peptide GPCRs Pathway (198897); Peptide Ligand-binding Receptors Pathway (106358)

Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

CX3CR1: Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. Defects in CX3CR1 are a cause of susceptibility to age- related macular degeneration type 12 (ARMD12). ARMD12 is a form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Receptor, cytokine; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: neuron projection; integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane. Molecular Function: protein binding; chemokine receptor activity; C-X3-C chemokine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; negative regulation of angiogenesis; cerebral cortex cell migration; viral reproduction; response to wounding; cellular defense response; chemotaxis; cell adhesion; macrophage chemotaxis; negative regulation of cell migration; microglial cell activation during immune response. Disease: Macular Degeneration, Age-related, 12; Coronary Heart Disease, Susceptibility To, 1; Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675