ELISA/assay

Rat Free cholesterol ELISA Kit

MBS732807

48-Strip-Wells

470 USD

ELISA Kit

Rat Free cholesterol ELISA Kit

Free cholesterol

FCHOL

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ?mol/L.

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This FC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY FC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FC antibody and an FC-HRP conjugate. The assay sample and buffer are incubated together with FC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FC concentration since FC from samples and FC-HRP conjugate compete for the anti-FC antibody binding site. Since the number of sites is limited, as more sites are occupied by FC from the sample, fewer sites are left to bind FC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FC concentration in each sample is interpolated from this standard curve

48-Strip-Wells

470 USD

96-Strip-Wells

675